hormone receptor fshr  (Bioss)

Journal: Scientific Reports

Article Title: Chronic unpredictable stress induces anxiety-like behavior and oxidative stress, leading to diminished ovarian reserve

doi: 10.1038/s41598-024-76717-y

Figure Lengend Snippet: CUS induce stress in female mice and impair reproductive function. ( A ) Representative images exhibiting the changes of female mice estrous cycle for 10 consecutive days. P, proestrus; E, estrus; M, metestrus; D, diestrus. ( B ) Serum reproductive hormone concentrations ( n = 8). AMH, anti-mullerian hormone; FSH, follicle stimulating hormone; E 2 , estradiol. ( C ) Representative HE staining images of mice ovarian tissues from CUS and control group ( n = 7). ( D ) The number of pups born (left) and the number of pups surviving (right) at three weeks were recorded for each pregnant female ( n = 5 in Control; n = 4 in CUS). ( E ) Protein expression analysis of AMH and FSHR in the ovaries ( n = 4). FSHR, follicle stimulating hormone receptor. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.

Article Snippet: The membranes were blocked with blocking buffer (Epizyme Biotech, PS108, Shanghai, China) for 30 min and incubated with the primary antibodies of AMH (abcam, ab229212), follicle stimulating hormone receptor (FSHR, proteintech, 22665-1-AP), BAX (proteintech, 50599-2-Ig), Bcl-2(proteintech, 68103-1-Ig), cytochrome complex (Cyt C, abcam, ab133504), beta actin (abcam, ab8226) and GAPDH (abcam, ab8245) at 4 °C for overnight.

Techniques: Staining, Control, Expressing

Journal: Poultry science

Article Title: Follicular fluid-derived exosomal HMOX1 promotes granulosa cell ferroptosis involved in follicular atresia in geese (Anser cygnoides).

doi: 10.1016/j.psj.2024.103912

Figure Lengend Snippet: Figure 2. GCs activity and sex steroid synthesis ability analysis of the GC layers of HSYFs and ASYFs. (A) GCs apoptosis in HSYFs and ASYFs were determined by TUNEL assay. The blue stain indicated the cell nucleus. Scale bar: 25 mm. (B) Relative content of sex steroid E2, P4 and mRNA expression levels of sex steroid synthesis-related genes CYP19A, HSD17B7, FSHR. Data are expressed as the means § SD. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: After that, purity was initially determined by an indirect immunofluorescence assay (IFA) to detect follicle GCs specific receptor, follicle-stimulating hormone receptor (FSHR) expression using anti-FSHR antibodies (1:500, Proteintech, Rosemont, IL, United States, L594-22665).

Techniques: Activity Assay, TUNEL Assay, Staining, Expressing

Journal: Poultry science

Article Title: Follicular fluid-derived exosomal HMOX1 promotes granulosa cell ferroptosis involved in follicular atresia in geese (Anser cygnoides).

doi: 10.1016/j.psj.2024.103912

Figure Lengend Snippet: Figure 6. Effect of HSYF-FF and ASYF-FF exosomes on ferroptosis in GCs. (A) Isolation and identification of goose GCs. Diagram of GCs (Scale bar: 100 mm), DAPI staining of cell nucleus, and fluorescent image of FSHR. (B) ASYF-FF exosomes were uptaken by GCs. (C) Cell prolifer- ation, relative content of MDA and the amount of Fe2+ of GCs treated with HSYF-Exo, ASYF-Exo, and ASYF-Exo+Fer-1, respectively. Data are expressed as the means § SD. Different lowercase letters indicate significant differences (P < 0.05).

Article Snippet: After that, purity was initially determined by an indirect immunofluorescence assay (IFA) to detect follicle GCs specific receptor, follicle-stimulating hormone receptor (FSHR) expression using anti-FSHR antibodies (1:500, Proteintech, Rosemont, IL, United States, L594-22665).

Techniques: Isolation, Staining